This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link ® Rapid Alexa Fluor ® 488 Labeling Kit. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated His-Tag (D3I1O) XP ® Rabbit mAb #12698. Molecular Probes™ Alexa Fluor™ 488 Protein Labeling Kit. SNAP-tag ® Purified Protein. Alexa Fluor 647 Protein Labeling Kit. For labeling . Name: Alexa Fluor 647 Protein Labeling Kit. Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or protein (20-100μg). Molecular Probes Alexa Fluor ® 633 Protein Labeling Kit pro-vides a convenient means to label proteins with our superior Alexa Fluor 633 dye. Thus, the Maxpar® labeling . Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. Kit [336-0010] Lightning-Link® Rapid Alexa Fluor® 647 Labeling Kit by Expedeon. Unlike other dyes, the fluorescence of Alexa Fluor®-conjugates is insensitive to pH between pH 4 . Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795) Specific References (38) Description: Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285) Specific References (39) Description: FITC Conjugation Kit (Fast) - Lightning-Link . Description: Molecular Probes Protein Labeling Kits provide a convenient means for attaching a fluorescent label or biotin to an antibody or a protein larger than 40 kDa Conjugates are ideal for multiple applications including flow cytometry fluorescent microscopy immunohistochemistry primary detection ELISAs immunocytochemistry FISH and more Kits are . In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes. A major advantage of Mix-n-Stain™ kits is our superior CF® dyes, many of which are brighter and more photostable than Alexa Fluor® dyes and other similar commercial dyes. Concentration Lot . Sign in or register to save this reagent to your favourites Search . Kit [A-20186] Alexa Fluor™ 647 Antibody Labeling Kit by Invitrogen Antibodies. Abs/Em Maxima: 395/545 nm. Format: Alexa Fluor 700 (A=675-700, E=723) Kit Type: Antibody Labeling Kit. This kit is optimized for labeling 1 mg of IgG antibody per reaction with Alexa Fluor 647. PEG spacers increase water solubility, enable efficient biolabeling in imaging and diagnostic R&D. Alexa Fluor dyes, Bodipy, Cyanine, Fluorescein, Rhodamine and Pyrene dyes. Andy Fluor™ 635 is water-soluable, highly fluorescent and pH-insensitive from pH 4 to pH 10. And generally when using Alexa Fluor™ dyes, higher degrees of labeling can be . Readilink(TM) protein labeling technology is the most robust and convenient tool for preparing fluorescent antibody conjugates used for fluorescence… EN. * 1. Alexa Fluor 633 dye . BP Fluor 647 (Alexa Fluor 647 equivalent) is a bright green-fluorescent dye optimal for use with the 633, 650 nm Argon laser. Comparable amounts of other . Product Details. ized with an Alexa Fluor 647 goat anti-chicken IgG (pseudocolored purple), goblet cells were labeled with an Alexa Fluor 594 conjugate of wheat germ agglutinin (red), and nuclei were labeled with anti-cdc6 peptide antibody and visualized with an Alexa Fluor 405 goat anti-mouse IgG (blue). And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved . Lightning-Link® Rapid Alexa Fluor 647 Antibody Labeling Kit Summary Applications/Dilutions Application Notes By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. These three dyes, BP Fluor 647, Cyanine . Thank you! However, because of its peak emission at 667 nm, Alexa Fluor . The Alexa Fluor 647 Monoclonal Antibody Labeling Kit provides a convenient means to label small amounts of monoclonal antibodies with the superior Alexa Fluor 647 dye. Technical Summary. Alexa Fluor® 647 Conjugation Kit / Alexa Fluor® 647 Labeling Kit ab269823 uses a simple and quick process for Alexa Fluor ® 647 labeling / conjugation of antibodies. CF®405L Dye. 1). The SAIVI™ Alexa Fluor™ 647 Antibody/Protein 1 mg-Labeling Kit provides a convenient means to label proteins with Alexa Fluor™ 647 near-IR emitting dye (Figure 1). . Alexa Fluor® 790 A20189 Protein Labeling Kit—Complete, easy-to-use kit designed for three reactions, each reaction optimized to label ~1 mg of protein. In the Microscale Protein Labeling Kits, the reactive dye contains a succinimidyl (NHS) ester moiety that reacts with primary amines of proteins to form stable dye-protein conjugates. Learn More. The purified protein was conjugated with Alexa Fluor® 647 under optimal conditions. SNAP-tag ® Purified Protein. Fluorescent dye is a fluorescent chemical compound that can re-emit light upon light excitation. Alexa Fluor 647 Protein Labeling Kit. Wish list; My account ; Shopping cart 0 0.00€ * Service/Help . 10 ug) and large quantities of primary antibodies with ease. This solution gives pH ~ 8.3. Alexa Fluor® 647 conjugates are the best choice of far-red emitting dyes for multiple labeling detection with a confocal microscope, and are also widely used in flow cytometry. Important Features of Microscale Protein Labeling Kits: Alexa Fluor®, Pacific Blue™, and Pacific Orange™ Antibody Labeling Kits | 2 Alexa Fluor® dyes produce protein conjugates that are typically brighter, more photostable, and less pH sensitive relative to the traditional dyes (AMCA, fluorescein/FITC, TRITC, etc.) Product Description. APEX® Antibody Labeling Kit— Complete . The Alexa Fluor® 488 Protein Labeling Kit contains everything that is needed to perform three separate labeling reactions and to purify the resulting conjugates. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Quick Protocol. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Lightning-Link Rapid Antibody Labeling kits enable direct labeling of antibodies, proteins, peptides or other biomolecules (see protocol for further information) and allow conjugations to be set up in seconds, and used within minutes (less than 20 minutes). Andy Fluor™ 647 is a bright, far-red fluorescent dye with excitation ideally suited to the 633 nm laser line. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. A 405 nm-excitable dye with orange fluorescence emission. The pHrodo iFL Microscale Protein Labeling Kit provides the reagents needed to easily label 3 microscale purified protein or antibody samples (20-100 µg) with pHrodo iFL Red or Green dye. Product Specs; Item Alexa Fluor 647 Protein Labeling Kit; Company Thermo Fisher Scientific; Catalog Number A20173; This product is no longer available on Biocompare. I was extremely impressed with this kit, as it is very well designed and easy to use. Important Features of Microscale Protein Labeling Kits: ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). 1278 Keywords: ReadiLink™ Rapid XFD647 Antibody Labeling Kit *XFD647 Same Structure to Alexa Fluor™ 647*, protocol, product, information, sheet, 1278 Created Date: 3/4/2021 6:03:06 PM Amount and volume of antibody for conjugation to Alexa Fluor ® 488 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Wilms Tumor Protein antibody [EPR23963-116] (ab283325) Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Wilms Tumor Protein with abab283325 at 1/100 (5.0 ug/ml) followed by a at dilution. Alexa Fluor 647 Protein Labeling Kit from Thermo Fisher Scientific. The Alexa Fluor 647 reactive dye has a succinimidyl ester moiety that reacts efficiently with pri-mary amines of proteins to form stable dye-protein conjugates. Menu Sign in or Register Search; Custom Suppliers; Data; Citations; Images; Listing; Contact; 2022 CiteAb Awards - Nominate now for the 2022 CiteAb Acquisition of the Year Award! Alexa Fluor™ 647 Protein Labeling Kit (Invitrogen™) Molecular Probes® Protein Labeling Kits provide a convenient means for attaching a fluorescent label (or biotin) to an antibody (or a protein larger than 40 kDa). The kit is designed for labeling and purifying 1 mg of protein per reaction, and has been optimized using 1 mg of IgG per conjugation reaction. Buy ReadiLink(TM) Rapid AF647 Antibody Labeling Kit *Rapid Alexa Fluor 647 Labeling*, item number: ABD-1278 from AAT Bioquest at Biomol! Extinction coefficient: 24,000. 100% Guarantee on every product. The dye has 4 sulfonate groups make it high water soluble and less aggregation in the aqueous solution. Several of our CF® dyes, such as CF®405M, CF®633, CF®640R, CF®647, and CF®680R, have been demonstrated to be ideal probes for super-resolution and single molecule imaging ( see references ). We offer Alexa Fluor 647 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. Lightning-Link (R) Rapid Alexa Fluor 700 Isotype Control [Alexa Fluor® 647] [337-0015] - Scans of the Absorbance and Fluorescence Emission of a Lightning-Link® Rapid Alexa Fluor® 700 Conjugate . The sulfonate groups make it high water soluble and less aggregation in the aqueous solution. Alexa Fluor™ 488 Protein Labeling Kit. BP Fluor 647 647 is used for protein and antibody labeling, or nucleic acid applications with high . The SAIVI Alexa Fluor 647 Antibody/Protein Labeling Kit provides the materials needed conveniently label an antibody or protein with amine-reactive far-red fluorescent Alexa Fluor 647 dye (ex/em 650/665 nm) for in vivo animal imaging applications. A20170 Alexa Fluor™ 633 632/647 Far Red[2] APC, Qdot ™ 655, BODIPY 630/650-X Cy5 A20173 Alexa Fluor ™ 647 650/668 Far Red[2] APC, Qdot™ 655, DDAO, DiD, DRAQ5™, TO-PRO ™-3, Cy5 , IRDye™ 650 Cy5 , APC A20171 Alexa Fluor™ 660 663/690 Far Red[2] Cy5.5 ™Cy5.5 A20172 Alexa Fluor™ 680 679/702 Infrared Qdot ™ 705, Cy5.5 , iRFP, IRDye . Order Details. Wish list; My account ; Shopping cart 0 0.00€ * Service/Help . Important Features of Alexa Fluor™ 647 Antibody Labeling Kit: With an excitation and emission maximum of 650/668 nm, Alexa Fluor™ 647 can be efficiently excited using a 633 or 635 nm Kr or He-Ne laser line and detected under standard APC/Cy5™ filters ; Labeled proteins typically ready to use typically in 90 min (∼15 min hands-on time) Useful for labeling 100μg of protein ; Optimized . Menu Sign in or Register Search ; Custom Suppliers; Data; Citations; Images; Listing; Contact; 2022 CiteAb Awards - Nominate now for the 2022 CiteAb Innovation Award! Sign in or register to save this reagent to your favourites Search (Z-25108) Zenon™ Alexa Fluor™ 647 Mouse . ®Add 100 μL of the antibody (from step 2) to the vial of Alexa Fluor dye. Invert to mix and incubate for 1 . AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. Note 2: . 2. Important Features of Alexa Fluor® 647 Antibody Labeling Kit: • With an excitation and emission maximum of 650/668 nm, Alexa Fluor® 647 can be efficiently excited using a 633 or 635 nm Kr or He-Ne laser line and detected under standard APC/Cy5® filters • Labeled proteins typically ready to use typically in 90 min (∼15 min hands-on time) 332-0030 is the same as the 3 x 10 ug size. Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines..Alexa Fluor 594 protein/antibody labeling kits. 3. The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the . Alexa Fluor 647 dye dashboard About Biocompare . Mouse intestine section labeled with four fl uorescent dyes. Alexa Fluor™ 647 Protein Labeling Kit Manufacturer: Molecular Probes™ A20173 Catalog No. Thermo Fisher alexa fluor 488 647 protein labeling kit Molecular Probes Protein Labeling Kits provide a convenient means for attaching a fluorescent label or biotin to an antibody or a protein larger than 40 kDa Conjugates are ideal for multiple applications including flow cytometry fluorescent microscopy immunohistochemistry primary detection ELISAs immunocytochemistry FISH and more Kits are . 1. It can also be used to conjugate other proteins or peptides. The Alexa Fluor® 647 Protein Labeling Kit is designed to allow you to quickly and easily label a protein with Alexa 647. Completely dissolve sodium bicarbonate in 1 mL of MilliQ water by vortexing or pipetting to make 1 M sodium bicarbonate solution. (A-20186) Alexa Fluor™ 647 Antibody Labeling Kit - Thermo Scientific - CiteAb. Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, FISH . Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. BP Fluor 647 is spectrally similar to Cy5, Cyanine 650. 332-0015 is the same as the 1 mg size. Filamentous actin was labeled with . we have the lightning link rapid alexa fluor 647 Antibody labeling kit and i was wondering if we can use it with IgG1 that contains 0.09% sodium azide phosphate-buffered solution. BP Fluor 647 (Alexa Fluor 647 ® equivalent) is a far-red bright and photostable fluorophore absorbed at 650 nm and emitted at 671 nm. Microscale Protein Labeling Kit— Complete, easy-to-use kit designed for three reactions, each reaction optimized to label ~20-100 µg of protein. ized with an Alexa Fluor 647 goat anti-chicken IgG (pseudocolored purple), goblet cells were labeled with an Alexa Fluor 594 conjugate of wheat germ agglutinin (red), and nuclei were labeled with anti-cdc6 peptide antibody and visualized with an Alexa Fluor 405 goat anti-mouse IgG (blue). The kit is designed for labeling and purifying 1 mg of protein per reaction, and has been optimized using 1 mg of IgG per conjugation reaction. Summary. Conjugate: Alexa Fluor 700. Alexa Fluor 647 Protein Labeling Kit. APEX Alexa Fluor 647 Antibody Labeling Kit from Thermo Fisher Scientific. We recently used this kit to label some purified antibodies that were made in the lab for flow cytometry. 300 400 500 600 700 800 900 Wavelength (nm) CF405L. 2. FAQs for Lightning-Link (R) Rapid Alexa Fluor 647 Kit (336-0030). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence . (labeling kit) iFluor™ 647 649 nm 664 nm Alternative to Cy5®, Alexa Fluor® 647 and DyLight™ 650 Strong fluorescence More photostable than Cy5® #1031 (SE, NH 2-reactive) #1055 (maleimide, SH-reactive) #1235 (labeling kit) iFluor™ 680 676 nm 695 nm Alternative to Cy5.5®, IRDye® 700, Alexa Fluor® 680 and . BP Fluor 647 is water soluble and pH-insensitive from pH 4 to pH 10. The exceptionally bright and photostable Alexa Fluor 633 dye was developed to be optimally excited with two increasingly popular excitation sources, the 633 nm line of the He-Ne laser and the 635 nm line of the red diode laser. The dye is water soluble and pH-insensitive from pH 4 to pH 10. Contact ; Technical . About Biocompare About . Comparable amounts of other proteins (>30 kDa) can also be labeled. The Alexa Fluor® 488 reac-tive dye (Figure 1) has a TFP ester moiety that is more stable in solution than the commonly used succinimidyl (NHS) ester. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8. This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in transfected cells. Menu Sign in or Register Search; Custom Suppliers; Data; Citations; Images; Listing; Contact ; 2022 CiteAb Awards - Nominate now for the 2022 CiteAb Environmental Initiative of the Year Award! The kits are available in four Alexa Fluor® colors (or biotin) and supply everything needed for three labeling and separation reactions. The Alexa Fluor 647 reactive dye has a succinimidyl ester moiety that reacts efficiently with primary amines of proteins . Protein Labeling Kits (For Alexa Fluor . HiLyte Fluor* 647 Labeling Kit-NH 2 is mainly used for the preparation of red fluorescence-labeled proteins, such as IgG, for immunostaining, and cellular proteins for tracing. In the Microscale Protein Labeling Kits, the reactive dye contains a succinimidyl (NHS) ester moiety that reacts with primary amines of proteins to form stable dye-protein conjugates. 1, right panel). Sign in or register to save this reagent to your favourites Search (336-0010) Lightning-Link® Rapid Alexa Fluor® 647 Labeling Kit Kit . Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. The kits are available in four Alexa Fluor™ colors (or biotin) and supply everything needed for three labeling and separation reactions. Mouse intestine section labeled with four fl uorescent dyes. Contact ; Technical Service ; FAQ . Pricing and Availability. An excellent choice for super-resolution imaging by SIM. Antibody and protein labeling kits. In the Microscale Protein Labeling Kits, the reactive dye contains a succinimidyl (NHS) ester moiety that reacts with primary amines of proteins to form stable dye-protein conjugates. NH 2-reactive HiLyteFluor 647, a component of this kit, has a succinimidyl group (NHS) that reacts with amino groups on proteins or other molecules (Fig. Pricing and Availability. 3. Alexa Fluor ® Antibody Labeling Kits. mend either the easy-to-use Alexa Fluor 647 Protein Labeling Kit (A20173), which is optimized for 1 mg samples of > 40 kDa pro-teins, or the Alexa Fluor 647 Monoclonal Antibody Labeling Kit (A20186), which is optimized for 100 µg samples of mono- or polyclonal antibodies. 332-0005 is the same as the 100 µg size.. Hi! Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. protein conjugate solution need be concentrated or freeze dried. See the characteristics of Alexa Fluor 647 dye and the range of antibody conjugates, kits, and other products related to this fluorophore. Add 50 μL of 1 M sodium bicarbonate to 0.5 mL of 2 mg/mL NT-Lys. TFP esters react efficiently with primary amines of proteins to form stable dye . Buy ReadiLink(TM) iFluor(TM) 647 Protein Labeling Kit *Microscale Optimized for Labeling 100 µg Antibody, item number: ABD-1235 from AAT Bioquest at Biomol! A significant advantage of long wavelength dyes such as Alexa Fluor® 647 is the low autofluorescence of biological specimens in this region of the spectrum. Lightning-Link . What does Alexa Fluor 594? Learn about our antibody labeling kits and their advantages. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot. Adjust the antibody concentration to 1.0 mg/mL, then add 1/10th volume of the 1 M sodium bicarbonate solution (from step 1). And generally when using Alexa Fluor™ dyes, higher degrees of labeling can be . Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8. This kit contains all the reagents necessary for labeling . The SAIVI™ Alexa Fluor® 647 Antibody/Protein 1 mg-Labeling Kit provides a convenient means to label proteins with Alexa Fluor® 647 near-IR emitting dye (Figure 1). Filamentous actin was labeled with . These dyes are bright, photostable and have minimal… EN. Description: Molecular Probes Protein Labeling Kits provide a convenient means for attaching a fluorescent label or biotin to an antibody or a protein larger than 40 kDa Conjugates are ideal for multiple applications including flow cytometry fluorescent microscopy immunohistochemistry primary detection ELISAs immunocytochemistry FISH and more Kits are . Alexa Fluor 647 is conjugated to NT-Lys using Alexa Fluor 647 protein labeling kit targeted to primary amines, following the manual of the manufacturer. Lightning-Link® Rapid Alexa Fluor 647 Antibody Labeling Kit Summary. 332-0010 is the same as the 3 x 100 ug size. Show more Hide. The Alexa Fluor 647 Protein Labeling Kit provides the materials needed to conveniently label an antibody or protein with amine-reactive far-red fluorescent Alexa Fluor 647 dye (ex/em 650/665 nm). Fluorophore label: Alexa Fluor™ 647 dye Reactive group: maleimide Reactivity: thiol groups on proteins and ligands, oligonucleotide thiophosphates Ex/Em of the conjugate: 651/671 nm Extinction coefficient: 265,000 cm-1M-1 Spectrally similar dyes: Cy5 Molecular weight: ∼1250 Typical Conjugation Reaction The protein should be dissolved at a concentration of 50-100μM in a suitable buffer (10 . A20173 $528.00 / Each Qty Add to cart Description Specifications Safety and Handling Description Molecular Probes™ Protein Labeling Kits provide a convenient means for attaching a fluorescent label (or biotin) to an antibody (or a protein larger than 40 kDa). 99. ReadiLink™ Rapid XFD647 Antibody Labeling Kit *XFD647 Same Structure to Alexa Fluor™ 647* Author: AAT Bioquest Subject: Protocol Sheet for Catalog No. Find ALEXA FLUOR ™- 647 Protein Labeling Kit and related products for scientific research at MilliporeSigma Molecular weight: ~1573. Each of the three vials of reactive dye provided in the kit is suffi . The Alexa Fluor 647 Monoclonal Antibody Labeling Kit provides a convenient means to label small amounts of monoclonal antibodies with the superior Alexa Fluor. Generate ready-to-use, Alexa Fluor-labeled antibodies even in the presence of contaminants with APEX Alexa Fluor Antibody Labeling kits. (Showing 1 - 1 of 1 FAQ). Kit [Z-25108] Zenon™ Alexa Fluor™ 647 Mouse IgG2a Labeling Kit by Invitrogen Antibodies. Important Features of Alexa Fluor™ 647 Antibody Labeling Kit: With an excitation and emission maximum of 650/668 nm, Alexa Fluor™ 647 can be efficiently excited using a 633 or 635 nm Kr or He-Ne laser line and detected under standard APC/Cy5™ filters Labeled proteins typically ready to use typically in 90 min (~15 min hands-on time) Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or protein (20-100 µg). Prepare a 1 M sodium bicarbonate solution by resuspending Component B in 1 mL of deionized water. Description: Molecular Probes Protein Labeling Kits provide a convenient means for attaching a fluorescent label or biotin to an antibody or a protein larger than 40 kDa Conjugates are ideal for multiple applications including flow cytometry fluorescent microscopy immunohistochemistry primary detection ELISAs immunocytochemistry FISH and more Kits are . So far in vitro transcription-based direct labeling of RNA probes with AF (Alexa Fluor®)-Dyes is rather inefficient.. We now offer HighYield T7 RNA Labeling Kits for efficient AF (Alexa Fluor®)-Dye labeling.. AF (Alexa Fluor®)-Dyes are hydrophilic fluorescent dyes with remarkable photostability however, when conjugated to normal 5-UTP, they show rather poor incorporation (Fig. The Alexa Fluor 647 protein Labeling Kit contains everything that is required to perform three separate labeling reactions and to purify the resulting conjugates. Description: Molecular Probes Protein Labeling Kits provide a convenient means for attaching a fluorescent label or biotin to an antibody or a protein larger than 40 kDa Conjugates are ideal for multiple applications including flow cytometry fluorescent microscopy immunohistochemistry primary . Generate antibody conjugates for fluorescent microscopy, flow cytometry, IHC, ICC, and more in just 15 minutes with Zip Alexa Fluor Rapid Antibody Labeling kits. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. and the Cy® dyes (Table 2). Add the . From start to finish, it takes less than three hours to label a protein and purify the resulting conjugate using the Alexa Fluor® 647 Protein Labeling Kit . Andy Fluor™ 647 can be used as a replacement for Alexa Fluor 647, DyLight 649, Cy5. Shop Shop Molecular Probes™ Alexa Fluor™ 647 Antibody Labeling Kit 1 kit Fluorescent Labelling Reagents for Proteins | Fisher Scientific Product Specs; Item APEX Alexa Fluor 647 Antibody Labeling Kit; Company Thermo Fisher Scientific; Catalog Number A10475; This product is no longer available on Biocompare. To conjugate an antibody to Alexa Fluor ® 647 using this kit: Description. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several bonds. ) and supply everything needed for three labeling and separation reactions for multiple applications, including flow.! Make 1 M sodium bicarbonate solution Zenon™ Alexa Fluor™ 647 mouse, primary detection, ELISAs, immunocytochemistry FISH... 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Secondary antibodies - Jackson Immuno < /a > alexa fluor 647 protein labeling kit Description 500 600 700 800 900 Wavelength ( nm ).. Add 50 μL of the 1 mg of IgG antibody per reaction with Alexa Fluor Secondary! Per reaction with Alexa Fluor 647® Secondary antibodies - Jackson Immuno < /a > Description... To conjugate other proteins or peptides account ; Shopping cart 0 0.00€ * Service/Help particular,.. To pH 10 ester moiety that reacts efficiently with primary amines of.! Milliq water by vortexing or pipetting to make 1 M sodium bicarbonate solution its peak emission at nm! Most popular amine reactive group for labeling proteins, in particular,.., or planar or cyclic molecules with several bonds convenient tool for preparing fluorescent antibody conjugates or probes for labeling... Is suffi in or register to save this reagent to your favourites Search ( Z-25108 Zenon™. Was extremely impressed with this kit is suffi is the same as the 3 x 100 ug size ideal multiple... Convenient tool for preparing fluorescent antibody conjugates or probes ( 336-0010 ) Lightning-Link® Alexa... Other dyes, bp Fluor 647 is water soluble and pH-insensitive from pH 4 to pH between pH to... Snap-Tag ® purified protein is water soluble and pH-insensitive from pH 4, dye... A=675-700, E=723 ) kit Type: antibody labeling, or planar or cyclic molecules with bonds..., ELISAs, immunocytochemistry, FISH favourites Search ( 336-0010 ) Lightning-Link® Rapid Fluor®! Unconjugated His-Tag ( D3I1O ) XP ® Rabbit mAb # 12698 647 is spectrally to! Photostable and have minimal… EN like other membrane-anchored ADAMs, ADAM10 consists of the (! ) Zenon™ Alexa Fluor™ dyes are optimized for labeling with the antibody ( from step 1 ), )! Each reaction optimized to label ~20-100 µg of protein kit by Invitrogen antibodies Bioquest & # x27 ; iFluor... Ester moiety that reacts efficiently with primary amines of proteins to form dye-protein... Labeling with the antibody labeling kits and their advantages //psichologyanswers.com/library/lecture/read/358125-what-color-is-alexa-fluor-647 '' > What color is Alexa Fluor 647 dye. Or succinimidyl ester moiety that reacts efficiently with primary amines of proteins to stable. Three dyes, Alexa Fluor™ dyes are brighter, more photostable, and new technologies for life....

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